ecm1  (Santa Cruz Biotechnology)

Journal: Scientific Reports

Article Title: H19 enhances pancreatic cancer proliferation and invasion by reducing miR-29c-5p’s inhibitory effects on ATF2/ECM1

doi: 10.1038/s41598-026-37632-6

Figure Lengend Snippet: Alterations in the H19/miR-29c-5p/ATF2/ECM1 Axis during Pancreatic Cancer Progression. ( A ) The differential mRNA expression of ATF2 and ECM1 in pancreatic cancer in the TCGA database; in pathological immunohistochemistry, the protein expression of ATF2 and ECM1 was significantly increased in the cancer nests of clinical patients compared to the adjacent tissues, * p < 0.05, ** p < 0.01, *** p < 0.001.). ( B ) The predicted binding sites of miR-29c-5p on the 3’UTR of ATF2 and the sequence illustration of the ATF2 binding site of the transcription factor. ( C ) In the TCGA database, miR-29c was negatively correlated with the expressions of ATF2 and ECM1. ( D ) Western blot verification of the protein levels of ATF2 and ECM1; knockdown of H19 decreased ATF2 and ECM1, while miR-29c-5p mimics exhibited a similar inhibitory effect. β-actin was utilized as a loading control, * p < 0.05, ** p < 0.01, *** p < 0.001.)

Article Snippet: Fig. 7 Expression and clinical significance of ATF2 and ECM1 genes in pancreatic cancer patients. ( A ) In the Human Protein Atlas database, low expression of ECM1 protein in pancreatic cancer indicates a better prognosis; in the TCGA database, it was found that low expression of ECM1 mRNA has a better survival prognosis; ECM1 mRNA has a good diagnostic effect, with an AUC area under the ROC curve of 0.978; ( B ) In the TCGA database, the AUC area under the ROC curve of ATF2 mRNA is 0.949, indicating a good diagnostic effect on pancreatic cancer; ( C ) In the immunohistochemistry of clinical sample pancreatic cancer pathological sections, high expression of ECM1 and ATF2 proteins was found; ( D ) Correlation between the mRNA expression of ATF2 and ECM1; ( E ) Subcellular localization of ATF2 and ECM1 in the Human Protein Atlas database.

Techniques: Expressing, Immunohistochemistry, Binding Assay, Sequencing, Western Blot, Knockdown, Control

Journal: Scientific Reports

Article Title: H19 enhances pancreatic cancer proliferation and invasion by reducing miR-29c-5p’s inhibitory effects on ATF2/ECM1

doi: 10.1038/s41598-026-37632-6

Figure Lengend Snippet: The H19/mir-29c-5p/ATF2/ECM1 axis promotes the growth of pancreatic cancer in a nude mouse model. ( A ) Tumor models were established by subcutaneous tumor formation experiments, and in vivo imaging of subcutaneous tumors in nude mice was performed; ( B ) Comparison of tumor weights in each group, * p < 0.05, ** p < 0.01, *** p < 0.001. ( C ) Immunohistochemical detection of ATF2 and ECM1 expression in tumor tissues of each group; The effects of H19 and miR-29c-5p on the nude mouse PC cell mode, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Fig. 7 Expression and clinical significance of ATF2 and ECM1 genes in pancreatic cancer patients. ( A ) In the Human Protein Atlas database, low expression of ECM1 protein in pancreatic cancer indicates a better prognosis; in the TCGA database, it was found that low expression of ECM1 mRNA has a better survival prognosis; ECM1 mRNA has a good diagnostic effect, with an AUC area under the ROC curve of 0.978; ( B ) In the TCGA database, the AUC area under the ROC curve of ATF2 mRNA is 0.949, indicating a good diagnostic effect on pancreatic cancer; ( C ) In the immunohistochemistry of clinical sample pancreatic cancer pathological sections, high expression of ECM1 and ATF2 proteins was found; ( D ) Correlation between the mRNA expression of ATF2 and ECM1; ( E ) Subcellular localization of ATF2 and ECM1 in the Human Protein Atlas database.

Techniques: In Vivo Imaging, Comparison, Immunohistochemical staining, Expressing

Journal: Scientific Reports

Article Title: H19 enhances pancreatic cancer proliferation and invasion by reducing miR-29c-5p’s inhibitory effects on ATF2/ECM1

doi: 10.1038/s41598-026-37632-6

Figure Lengend Snippet: Expression and clinical significance of ATF2 and ECM1 genes in pancreatic cancer patients. ( A ) In the Human Protein Atlas database, low expression of ECM1 protein in pancreatic cancer indicates a better prognosis; in the TCGA database, it was found that low expression of ECM1 mRNA has a better survival prognosis; ECM1 mRNA has a good diagnostic effect, with an AUC area under the ROC curve of 0.978; ( B ) In the TCGA database, the AUC area under the ROC curve of ATF2 mRNA is 0.949, indicating a good diagnostic effect on pancreatic cancer; ( C ) In the immunohistochemistry of clinical sample pancreatic cancer pathological sections, high expression of ECM1 and ATF2 proteins was found; ( D ) Correlation between the mRNA expression of ATF2 and ECM1; ( E ) Subcellular localization of ATF2 and ECM1 in the Human Protein Atlas database.

Article Snippet: Fig. 7 Expression and clinical significance of ATF2 and ECM1 genes in pancreatic cancer patients. ( A ) In the Human Protein Atlas database, low expression of ECM1 protein in pancreatic cancer indicates a better prognosis; in the TCGA database, it was found that low expression of ECM1 mRNA has a better survival prognosis; ECM1 mRNA has a good diagnostic effect, with an AUC area under the ROC curve of 0.978; ( B ) In the TCGA database, the AUC area under the ROC curve of ATF2 mRNA is 0.949, indicating a good diagnostic effect on pancreatic cancer; ( C ) In the immunohistochemistry of clinical sample pancreatic cancer pathological sections, high expression of ECM1 and ATF2 proteins was found; ( D ) Correlation between the mRNA expression of ATF2 and ECM1; ( E ) Subcellular localization of ATF2 and ECM1 in the Human Protein Atlas database.

Techniques: Expressing, Diagnostic Assay, Immunohistochemistry